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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/01/2022 |
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Data da última atualização: |
20/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
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Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
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ISSN: |
1999-4915 |
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DOI: |
https://doi.org/10.3390/v13122498 |
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Idioma: |
Inglês |
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Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02107naa a2200217 a 4500
001 2139210
005 2022-01-20
008 2021 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $ahttps://doi.org/10.3390/v13122498$2DOI
100 1 $aLEASTRO, M. O.
245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource]
260 $c2021
520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.
650 $aVírus
700 1 $aVILLAR-ÁLVAREZ, D.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tViruses$gv.13, n.12, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Classificação |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
03/12/2012 |
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Data da última atualização: |
23/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ASCHERI, J. L. R.; TAKEITI, C. Y.; CARVALHO, C. W. P. de; GALDEANO, M. C.; SOUZA, V. F. de; SILVA, E. C. de B.; REGO, J. de O. |
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Afiliação: |
JOSE LUIS RAMIREZ ASCHERI, CTAA; CRISTINA YOSHIE TAKEITI, CTAA; CARLOS WANDERLEI PILER DE CARVALHO, CTAA; MELICIA CINTIA GALDEANO, CTAA; VALÉRIA FRANÇA DE SOUZA, UFRRJ; EMERSON COSME DE BRITO SILVA, UFRRJ; JANAINA DE OLIVEIRA REGO, UFRRJ. |
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Título: |
Uso da extrusão no desenvolvimento de uma farinha mista instantânea de arroz e casca e albedo de maracujá, para uso como alimento funcional: parte II. Elaboração de biscoito doce e avaliação sensorial. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON MEDICINAL AND NUTRACEUTICAL PLANTS, 3.; CONFERENCE OF NATIONAL INSTITUTE OF SCIENCE & TECHNOLOGY FOR TROPICAL FRUITS, 3., 2012, Aracaju. Annals... Aracaju: SBCTA-SE, 2012. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Coprodutos; Extrusão termoplástica; Fibra alimentar. |
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Thesagro: |
Maracujá. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71486/1/2012-207.pdf
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Marc: |
LEADER 00941nam a2200217 a 4500
001 1940962
005 2016-02-23
008 2012 bl uuuu u00u1 u #d
100 1 $aASCHERI, J. L. R.
245 $aUso da extrusão no desenvolvimento de uma farinha mista instantânea de arroz e casca e albedo de maracujá, para uso como alimento funcional$bparte II. Elaboração de biscoito doce e avaliação sensorial.
260 $aIn: INTERNATIONAL SYMPOSIUM ON MEDICINAL AND NUTRACEUTICAL PLANTS, 3.; CONFERENCE OF NATIONAL INSTITUTE OF SCIENCE & TECHNOLOGY FOR TROPICAL FRUITS, 3., 2012, Aracaju. Annals... Aracaju: SBCTA-SE, 2012. 1 CD-ROM.$c2012
650 $aMaracujá
653 $aCoprodutos
653 $aExtrusão termoplástica
653 $aFibra alimentar
700 1 $aTAKEITI, C. Y.
700 1 $aCARVALHO, C. W. P. de
700 1 $aGALDEANO, M. C.
700 1 $aSOUZA, V. F. de
700 1 $aSILVA, E. C. de B.
700 1 $aREGO, J. de O.
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Embrapa Agroindústria de Alimentos (CTAA) |
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